404 error when profiling

When I try and profile an ASP.NET application. It take a little time and then give me a 404 error. When I stop profiling it has 2 lines
System.Web.Hosting.AppManagerAppDomainFactor..ctor()
System.AppDomain.SetupDoamin(bool allowRedirects, string path, string configFile)

I got to a url like http://myapplication.mycompany.com:8013/default.aspx

So did it crash while trying to load the appdomain?
Any suggestions about what I can do?

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